General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen ETA Endothelin Receptor Membrane Preparation, 1 vial. (HTS070M)
The ChemiScreen ETA Endothelin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of EDNRA surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay ETA Endothelin Receptor Frozen Cells, 2 Vials of Cells. (HTS070RTA)
The Ready-to-Assay ETA Endothelin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. endothelin) based activation of EDNRA GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses EDNRA alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of EDNRA, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CXCR3 Chemokine Receptor Stable Cell Line, 2 vials. (HTS003C)
The ChemiScreen CXCR3 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay µ Opioid Receptor Frozen Cells, 2 Vials of Cells. (HTS101RTA)
The Ready-to-Assay µ Opioid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. opioids) based activation of OPRM1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRM1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of OPRM1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen µ Opioid Receptor Membrane Preparation, 1 vial. (HTS101M)
The ChemiScreen µ Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRM1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen EP1 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS099C)
The ChemiScreen EP1 Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PrecisION hGlyRA1 Recombinant Stable Cell Line, 2 vials. (CYL3056)
The PrecisION hGlyRA1 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying GlyRA1 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses GlyRA1 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx ChemiScreen Motilin Receptor Membrane Preparation, 1 vial. (HTS121M)
The ChemiScreen Motilin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MLNR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen M2 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS115M)
The ChemiScreen M2 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen M3 Muscarinic Acetylcholine Receptor Stable Cell Line, 2 vials. (HTS116C)
The ChemiScreen M3 Muscarinic Acetylcholine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen 5-HT6 Serotonin Receptor Stable Cell Line, 2 vials. (HTS111C)
The ChemiScreen 5-HT6 Serotonin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR6 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR6 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HTR6, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen LPA1 Lysophopholipid Receptor Stable Cell Line, 2 vials. (HTS089C)
The ChemiScreen LPA1 Lysophopholipid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. LPA) based activation of LPAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LPAR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of LPAR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen FPRL1 N-Formylpeptide Receptor Membrane Preparation, 1 vial. (HTS056M)
The ChemiScreen FPRL1 N-Formylpeptide Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of FPR2 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay BB2 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS084RTA)
The Ready-to-Assay BB2 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin) based activation of GRPR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRPR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GRPR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen BB2 Bombesin Receptor Stable Cell Line, 2 vials. (HTS084C)
The ChemiScreen BB2 Bombesin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. bombesin) based activation of GRPR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRPR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of GRPR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.